Gene editing and modification: Combining AI and molecular biology technologies, gene editing and modification of model engineering bacteria such as Escherichia coli are carried out to increase the production of HMOs and the adaptability of host bacteria. Microfluidic high-throughput screening: Utilizing microfluidic high-throughput screening technology to achieve rapid screening of large-scale bacterial libraries and efficient construction of bacterial strains.

Through precise gene editing and intelligent data analysis, efficiently and accurately design and optimize the performance of microbial strains.

By using microfluidic technology, more suitable fermentation micro conditions for bacterial strains were screened, and then the fermentation process was gradually scaled up from micro volume shake bottle-5L-50L-100L-1000L small-scale to pilot scale, exploring the effects of different temperatures and medium compositions on the target product and more stable fermentation process conditions that are closer to industrialization.

The purification technology of HMOs mainly includes decolorization and desalination systems and chromatographic separation systems. The HMOs fermentation broth after decolorization and desalination is subjected to a simulated moving bed device filled with a specially designed homogeneous chromatographic separation resin to obtain the target product LNT or LNnT. This device utilizes the principle that different sugars have different affinities for resins to separate different sugars in the fermentation broth, and can also perform secondary chromatographic separation to further improve the purity of the feed solution. The purity of LNT and LNnT obtained using this purification technology reaches over 92%.